Fig. 4: SIRT3 directly mediates the deacetylation of ACSL4. | Cell Death & Disease

Fig. 4: SIRT3 directly mediates the deacetylation of ACSL4.

From: HAT1/HDAC2 mediated ACSL4 acetylation confers radiosensitivity by inducing ferroptosis in nasopharyngeal carcinoma

Fig. 4

A IP assay was used to detect the interaction between ACSL4 and SIRT3 in NPC cells. B His-SIRT3 and Flag-ACSL4 plasmids were transfected into 293 T cells, and the interaction between ACSL4 and SIRT3 was detected by IP assay. C The subcellular localization of ACSL4 and SIRT3 was detected by IF assay. D Molecular docking analysis of the interaction between ACSL4 and SIRT3. E After SIRT3 was overexpressed in NPC cells, the acetylation and protein expression of ACSL4 were detected by IP assay. F HONE1 cells were treated with 3-TYP (50 μM) for 24 h, the acetylation of ACSL4 were detected by IP assay. G SIRT3-WT or SIRT3-H248Y plasmids were transfected into SUNE1 cells, and the acetylation and protein expression of ACSL4 were detected by IP assay. Blank plasmid (vec), negative control (NC).

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