Fig. 5: HAT1 directly mediates the acetylation of ACSL4. | Cell Death & Disease

Fig. 5: HAT1 directly mediates the acetylation of ACSL4.

From: HAT1/HDAC2 mediated ACSL4 acetylation confers radiosensitivity by inducing ferroptosis in nasopharyngeal carcinoma

Fig. 5

A Protein silver staining of IP by ACSL4 antibody enrichment. B Acetyltransferase in the protein mass spectrometry data. C His-HAT1 and Flag-ACSL4 plasmids were transfected into 293 T cells, and the interaction between ACSL4 and HAT1 was detected by IP. D IP was used to detect the interaction between ACSL4 and HAT1 in NPC cells. E The subcellular localization of ACSL4 and HAT1 was detected by IF. F Molecular docking analysis of the interaction between ACSL4 and HAT1. G HAT1 was knocked down in NPC cells, the acetylation of ACSL4 were detected by IP assay. H GSE12452 dataset was used to analyze the expression of HAT1. I The protein expression of HAT1 in NPC proteomics data. J, K The representative IHC images of HAT1 and ACSL4 in clinical samples and statistical analysis by IHC scores. L Correlation analysis for the expression of HAT1 and ACSL4 by IHC scores. Negative control (NC). The error line is expressed as mean ± SD. *p < 0.05, **p < 0.01.

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