Fig. 9: K383 acetylation of ACSL4 enhances radiosensitivity by inducing ferroptosis of NPC. | Cell Death & Disease

Fig. 9: K383 acetylation of ACSL4 enhances radiosensitivity by inducing ferroptosis of NPC.

From: HAT1/HDAC2 mediated ACSL4 acetylation confers radiosensitivity by inducing ferroptosis in nasopharyngeal carcinoma

Fig. 9

The indicated stable cell lines were used. A CCK8 assay was used to detect the cell viability treated with RSL3 (5 μM) or with both RSL3 (5 μM) and Fer-1 (2 μM) for 24 h. B After treated with RSL3 (5 μM) for 24 h, lip-ROS levels were detected by flow cytometry. C Cells were treated with RSL3 (5 μM) for 24 h, the mitochondrial morphology was observed by TEM. D The radioresistance of NPC cells (3 × 103 cells/well) was detected by colony formation assay under different doses (0, 1, 2, 4, 6 Gy) of IR and treatment with RSL3 (5 μM). 2 × 106 SUNE1-shACSL4-WT or SUNE1-shACSL4-K383R cells were injected subcutaneously into nude mice and randomly divided into non-IR group and IR group, and then divided into DMSO treatment group and RSL3 treatment group (n = 6). E Tumor size, F tumor volume and G tumor weight was measured. H IHC was used to detect the expression of ACSL4, Ki67 and 4-HNE in tumor tissues. Blank plasmid (vec). The error line is expressed as mean ± SD. *p < 0.05, **p < 0.01, ***p < 0.001.

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