Fig. 2: DNA methylation contributes to PLOD3 over expression. | Cell Death & Disease

Fig. 2: DNA methylation contributes to PLOD3 over expression.

From: Hypomethylation induced overexpression of PLOD3 facilitates colorectal cancer progression through TM9SF4-mediated autophagy

Fig. 2

A CpG islands were predicted in the PLOD3 promoter sequence. B TCGA database was employed to evaluate the correlation between PLOD3 mRNA expression and DNA methylation levels. C methylation-specific PCR(MSP) was performed to detect the PLOD3 methylation levels in eight randomly selected pairs of CRC tissues and corresponding adjacent tissues. M, methylated; U, unmethylated. D bisulfite sequencing PCR(BSP) was conducted to assessed the DNA methylation status of 8 randomly selected pairs of CRC tissues and corresponding adjacent tissues. E Increasing expression of PLOD3 protein levels in CRC cells incubated with different concentration of 5-Aza-dC. F Increasing expression of PLOD3 mRNA levels in CRC cells incubated with different concentration of 5-Aza-dC. All data are shown as the mean ± SD of three independent biological replicates. *P < 0.05, ****P < 0.0001 and P > 0.05, not significant (n.s.).

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