Fig. 6: ROS and mPTP were involved in CKMT1-mediated IECs apoptosis. | Cell Death & Disease

Fig. 6: ROS and mPTP were involved in CKMT1-mediated IECs apoptosis.

From: CKMT1 deficiency contributes to mitochondrial dysfunction and promotes intestinal epithelial cell apoptosis via reverse electron transfer-derived ROS in colitis

Fig. 6

A Flow cytometry analysis of the opening of mPTP (Calcein fluorescence) in Lovo cells. B Flow cytometry analysis of the effect of NAC pretreatment (5 mM for 3 h) on the opening of mPTP induced by TNF-α in siCK cells. C Flow cytometry analysis of the effect of CsA pretreatment (1 μM for 3 h) on TNF-α-induced mtROS generation in siCK cells. D Assessment of cytochrome c, ATP5A, and SPG7 in cytosolic or mitochondrial fraction of siCK cells after apoptosis induction via Western blotting. E Assessment of AIF and BAX expressions after apoptosis induction via Western blotting. F Assessment of the levels of apoptosis-related proteins in siCK cells upon NAC and CsA pretreatment via western blotting (n = 4). All flow cytometry experiments were repeated three times. *P < 0.05; **P < 0.01; ***P < 0.001.

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