Fig. 6: Lipid droplet (LD) formation is not required for OA to inhibit ferroptosis.
From: Monounsaturated fatty acids promote cancer radioresistance by inhibiting ferroptosis through ACSL3
A Oil red O staining of ACSL3 KO SW837-RR and CMT93-RR cells with or without 20 nM OA exposure. Scale bars, 50 μm. B The overexpression efficiency of ACSL3 in SW837 and CMT93 cells was determined by western blotting. C Images of Oil Red O stained ACSL3-OE SW837 and CMT93 cells exposed to DGATis (a combination of T863 (20 μM) and PF-06424439 (10 μM)). D CCK-8 assays were performed to determine the effects of 20 nM OA and DGATis (a combination of T863 (20 μM) and PF-06424439 (10 μM)) on the viability of ACSL3-OE SW837 and CMT93 cells. Quantitative analyses of lipid peroxidation levels (E) and the GSH/GSSG ratio (F) in OE ACSL3 SW837 and CMT93 cells treated with or without OA (20 nM) and DGATis. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
