Fig. 7: shACSL3-AAV combined with IKE significantly represses radioresistance in vivo.
From: Monounsaturated fatty acids promote cancer radioresistance by inhibiting ferroptosis through ACSL3
A CCK-8 assays were performed to determine the effects of 50 µM IKE and shACSL3-AAV (450,000 genome copies/cell) on the viability of CMT93-RR cells. B The treatment schedule for the CMT93-RR syngeneic mouse model. shACSL3-AAV was administered intratumorally at a dose of 1.8 × 1011 viral particles per animal. Injections were performed once every two days for a total of 6 times. Mice were treated with PBS, 30 mg/kg IKE, or shACSL3-AAV (1.8 × 1011 viral particles per animal) as a single agent or in combination. C Representative images of tumors. Tumor volume (D), tumor weights (E), and body weights of the mice (F). (n = 5) G The expression Ki67 and 4-HNE in the different treatment groups was measured by IHC. H Schematic illustration demonstrating that OA regulates ferroptosis and radioresistance in an ACSL3-dependent manner. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.
