Fig. 1: Low expression of PER2 in OSCC and positive correlation with cuproptosis. | Cell Death & Disease

Fig. 1: Low expression of PER2 in OSCC and positive correlation with cuproptosis.

From: PER2 interaction with HSP70 promotes cuproptosis in oral squamous carcinoma cells by decreasing AKT stability

Fig. 1

TCGA database and Pearson correlation analysis of the differences (A) and correlation (B) of the expression of PER2 and 13 cuproptosis genes in OSCC compared with normal tissues. RT-qPCR assay and Pearson correlation analysis of 26 pairs of human OSCC and paired adjacent normal tissues for differences in expression (C) and correlation (D) of PER2 and 13 cuproptosis genes. RT-qPCR assay and Pearson correlation analysis of the differences (E) and correlation (F) of the expression of PER2 and 13 cuproptosis genes in SCC25 cells compared with HOK cells. G Venn diagrams take intersection results (correlation factor threshold >0.15). RT-qPCR detection of differential expression of PER2 and 3 cuproptosis genes, DLAT, PDHB, and SLC31A1 mRNA, in CAL27 and TSCCA cells (H) and correlation analysis by Pearson’s method (I). Western blotting to detect the expression of PER2, DLAT, PDHB, and SLC31A1 proteins in CAL27, TSCCA, and SCC25 cells (J) and Pearson correlation analysis (K). IHC assay of PER2, DLAT, PDHB, and SLC31A1 protein expression in OSCC tissues (n = 26, scale bars = 50 μm) (L) and Pearson correlation analysis (M). All data represent three replicate independent experiments. Data are presented as mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001. ANT adjacent normal tissue.

Back to article page