Fig. 6: NAT10 promotes the progression of ccRCC by regulating NFE2L3. | Cell Death & Disease

Fig. 6: NAT10 promotes the progression of ccRCC by regulating NFE2L3.

From: NAT10 promotes the progression of clear cell renal cell carcinoma by regulating ac4C acetylation of NFE2L3 and activating AKT/GSK3β signaling pathway

Fig. 6

a Relative expression of NAT10 in KIRC and normal tissues in GEO datasets. b Kaplan-Meier survival analysis of NFE2L3 expression in ccRCC patients (n = 261, p < 0.0001, log-rank test). c QRT-PCR was used to detect NFE2L3 mRNA levels in 12 pairs of ccRCC cancer tissues and adjacent tissues. d Western blot analysis of NFE2L3 knockdown efficiency in 786-O and A498 cells. e The knockdown effect of NFE2L3 small interference RNA in 786-O and A498 cells by qRT-PCR. f Left: EdU assayed evaluating cell proliferation. Right: Quantitative analysis of EdU+ proliferating cells. Scale: 50 μm. g CCK8 assayed evaluating cell proliferation. h Left: Transwell assayed evaluating cell migration. Right: Quantitative analysis of migrating cells. Scale: 50 μm. i Flow cytometry showed that G0/G1 phase cycle arrest occurred after NFE2L3 knockdown. j The proliferation ability of 786-O and A498 cells after overexpression of NAT10 and transfection of NFE2L3 siRNAs was detected by CCK8 assays. k Left: The migration ability of 786-O and A498 cells after overexpression of NAT10 and transfection of NFE2L3 siRNAs was detected by Transwell assays. Right:quantitative analysis of migrating cells. Scale: 50μm.

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