Fig. 4: ELANE enhances tumor progression by acting on the tumor cell to promote the activation of the Hippo pathway in tumor cells. | Cell Death & Disease

Fig. 4: ELANE enhances tumor progression by acting on the tumor cell to promote the activation of the Hippo pathway in tumor cells.

From: Downregulation of tropomyosin 2 promotes the progression of lung adenocarcinoma by regulating neutrophil infiltration through neutrophil elastase

Fig. 4

A A549 and H1975 cells were treated with different concentrations of exogenous ELANE recombinant protein to detect the cell cycle, apoptosis, and EMT-producing protein levels. B–F CM-A-Neu-mediated tumor cells A549 and H1975 were treated with or without ELANE inhibitor HY-15651. The differences of proliferation (B), growth (C), cell cycle (D), apoptosis (E), and invasion (F) (100×) were detected. G Volcano map representing the differentially expressed proteins in A549 cells treated with CM-A-Neu vs A549 cells treated with CM-H-Neu. H The TPM2 protein level of A549 cells treated with CM-A-Neu and CM-H-Neu was detected. I GO and KEGG enrichment analysis based on the 362 downregulated proteins in A549 cells treated with CM-A-Neu. J CM-A-Neu-mediated tumor cells A549 were treated with or without ELANE inhibitor HY-15651 to detect the Hippo pathway, Cycle, apoptosis, and EMT-maker protein levels. p values were obtained by repeated measures two-tailed unpaired t-test; ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05.

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