Fig. 6: Downregulation of TPM2 promotes neutrophil recruitment to tumor sites by activating the ELANE-ERK1/2-IL1β/IL8 axis of neutrophils. | Cell Death & Disease

Fig. 6: Downregulation of TPM2 promotes neutrophil recruitment to tumor sites by activating the ELANE-ERK1/2-IL1β/IL8 axis of neutrophils.

From: Downregulation of tropomyosin 2 promotes the progression of lung adenocarcinoma by regulating neutrophil infiltration through neutrophil elastase

Fig. 6

A, B Migration of Peripheral blood-derived neutrophils recruited by conditioned medium of neutrophils obtained from A-neu and H-neu pretreated with TPM2-overexpressed A549 cells or TPM2-konckdown HCC827 cells. C, D A-neu and H-neu IL1β and IL8 secretion levels after pretreated with TPM2-overexpressed A549 cells or TPM2-konckdown HCC827 cells. E Migration of Peripheral blood-derived neutrophils recruited by CM-A-neu+ TPM2 with and/or IL1β and IL8-neutralizing antibodies. F Migration of Peripheral blood-derived neutrophils recruited by CM-A-neu+ TPM2 with ELANE inhibitor Alvelestat. G The expression of ERK, JNK1, NF-kB, PI3K, and Akt pathway proteins were detected after neutrophils were pretreated with different concentrations of ELANE. H, I After pretreatment of neutrophils with TPM2-overexpressed A549 cells/TPM2-konckdown HCC827 cells and/or 20 μmol/l ELANE inhibitor (Alvelestat) to detect p-ERK1/2 and ERK1/2 expression. J, K Protein levels of IL1β and IL8 in A-neu after pretreated with TPM2-overexpressed A549 cells or TPM2-konckdown HCC827 cells combined with 20 μmol/l ELANE inhibitor (Alvelestat). p values were obtained by repeated measures two-tailed unpaired t-test; ****p < 0.0001; ***p < 0.001; **p < 0.01; *p < 0.05; ns not significant.

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