Fig. 3: UHRF1 regulates the expression of SFRP5 through DNA methylation. | Cell Death & Disease

Fig. 3: UHRF1 regulates the expression of SFRP5 through DNA methylation.

From: UHRF1-mediated epigenetic reprogramming regulates glycolysis to promote progression of B-cell acute lymphoblastic leukemia

Fig. 3

A The heat map illustrates DEGs between the KO group and the NT group in the Nalm6 cell line. B In the KO group, the integration of up-regulated DEGs and demethylated DMR was analyzed by Wayne diagram. C Top 10 DEGs in the Intersection of Venn Diagram. D The mRNA expression level of SFRP5 after UHRF1 knockout and restoration. E and F The protein expression level of SFRP5 after UHRF1 knockout and restoration. G The CHIP-qPCR results indicate that the signal intensity of SFRP5 is stronger compared to the IgG control group. H The box plot demonstrates the upregulation of DNA methylation levels of SFRP5 following UHRF1 knockout. I Binding-dissociation curves between SFRP5 and WNT5A at different concentrations in BLI assay. J. Immunofluorescence staining reveals an increase in the protein levels of SFRP5 following UHRF1 knockout, and co-localization of SFPR5 and WNT5A on the cell membrane. The magnification of the images is ×850 for the full blots. N6: Nalm6.

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