Fig. 4: UHRF1 regulates glycolysis via the P38 MAPK-HK2 signaling axis. | Cell Death & Disease

Fig. 4: UHRF1 regulates glycolysis via the P38 MAPK-HK2 signaling axis.

From: UHRF1-mediated epigenetic reprogramming regulates glycolysis to promote progression of B-cell acute lymphoblastic leukemia

Fig. 4

A The bar graph presents KEGG enrichment analysis results for DEGs in the transcriptome. B NRM-based metabolic analysis was conducted to investigate intracellular metabolites. C Representative ECAR profiles of Nalm6 and Reh cells following UHRF1 knockout in the glycolytic rate assay. D Detection of lactate content in the cell culture medium. E Representative ECAR profiles of Nalm6 and Reh cells following UHRF1 restoration in the extracellular acidification assay. F The mRNA expression level of HK2 was detected by RT-qPCR. G Measurement of HK2 protein expression levels based on the western bot. H The phosphorylation level of P38 protein was assessed utilizing a western blot. I The mRNA expression level of HK2 in B-ALL cell lines treated with P38 inhibitor (SB203580). J Comparing the expression levels of HK2 protein between groups treated with SB203580 and control groups. K The lactate content in the cell culture medium was decreased in B-ALL cell lines treated with SB203580. L Representative ECAR profiles of Nalm6 and Reh's cells following P38 inhibitor treatment in the glycolytic rate assay. N6 Nalm6, SB SB203580.

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