Fig. 5: SFRP5 downregulates glycolysis via the WNT5A-P38 MAPK-HK2 signaling axis. | Cell Death & Disease

Fig. 5: SFRP5 downregulates glycolysis via the WNT5A-P38 MAPK-HK2 signaling axis.

From: UHRF1-mediated epigenetic reprogramming regulates glycolysis to promote progression of B-cell acute lymphoblastic leukemia

Fig. 5

A The mRNA expression level of HK2 and SFRP5 upon overexpression of SFRP5 in B-ALL cell lines. B The protein expression level of SFRP5 upon overexpression of SFRP5 in B-ALL cell lines. C The cell proliferation was detected using MTS methods. D The cell cycle of leukemia cells was detected using flow cytometry. E The lactate content in the cell culture medium was decreased in the SFRP5 overexpression group. F Representative ECAR profiles of Nalm6 and Reh cells following SFRP5 overexpression in the extracellular acidification assay. G Comparing the expression levels of phosphorylated P38 protein and HK2 protein between groups overexpressing SFRP5 and control groups. H The mRNA expression level of HK2 in B-ALL cell lines treated with WNT5A inhibitor (Box 5). I Comparing the expression levels of phosphorylated P38 protein and HK2 protein between groups treated with BOX5 and control groups. J The lactate content in the cell culture medium was decreased in B-ALL cell lines treated with Box 5. K Representative ECAR profiles of Nalm6 and Reh cells following BOX5 treatment in the glycolytic rate assay. N6 Nalm6, BX BOX5.

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