Fig. 5: Hypoxia condition induced lactate secretion of CCA cells, further facilitated SC dedifferentiation. | Cell Death & Disease

Fig. 5: Hypoxia condition induced lactate secretion of CCA cells, further facilitated SC dedifferentiation.

From: Single-cell analysis reveals that GFAP+ dedifferentiated Schwann cells promote tumor progress in PNI-positive distal cholangiocarcinoma via lactate/HMGB1 axis

Fig. 5

A The protein levels of HIF-1α in CCLP1 and HUCCT1 cells under normoxia and hypoxia conditions. B The protein levels of GFAP in ipNF95.6 cells receiving supernatants from CCA cells under normoxia and hypoxia conditions. C Detection of lactate in the supernatants of CCLP1 and HUCCT1 cells by lactate assay kit. D Detection of lactate in positive and negative nerve tissues for SC dedifferentiation markers (GFAP and NCAM1) in the Zhengzhou-dCCA cohort by lactate assay kit. E Expression levels of 10 dedifferentiation-related markers in SCs with high and low lactate metabolism. F The cytotoxic activity of lactate was measured using the MTT cell viability assay in ipNF95.6 cells. G Detection of mRNA expression levels of seven dedifferentiation-related markers in ipNF95.6 cells by RT‑qPCR. H, I Western blotting assays detecting protein levels of SC dedifferentiation members in ipNF95.6 (H) and RSC96 cells (I). *P < 0.05, **P < 0.01, ***P < 0.001.

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