Fig. 6: Cancer cell-derived lactate upregulated HMGB1 in SCs and HMGB1 further promoted carcinogenic behaviors in CCA cells. | Cell Death & Disease

Fig. 6: Cancer cell-derived lactate upregulated HMGB1 in SCs and HMGB1 further promoted carcinogenic behaviors in CCA cells.

From: Single-cell analysis reveals that GFAP+ dedifferentiated Schwann cells promote tumor progress in PNI-positive distal cholangiocarcinoma via lactate/HMGB1 axis

Fig. 6

A The percentage of GFAP-positive cells is positively correlated with the proportion of cells with high levels of HMGB1. B The correlation between IHC expression of SC dedifferentiation markers (GFAP and NCAM1) and IHC scores of HMGB1 in the Zhengzhou-dCCA cohort. C The protein level of HMGB1 in ipNF95.6 cells receiving supernatants from cancer cells under normoxia and hypoxia conditions. D The observation of HMGB1 protein levels in ipNF95.6 cells using immunofluorescence. E The protein levels of HMGB1 in CCLP1 and HUCCT1 cells. F, G Wound healing assay (F) and trans-well invasion assay (G) were performed respectively to assess the mobility and invasion of cancer cells. H The xenograft tumor model was established with a mixture of CCLP1 cells and ipNF95.6 cells. The arrows indicate the subcutaneous tumor. I Tumor volumes were documented every 3 days. *P < 0.05, **P < 0.01, ***P < 0.001.

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