Fig. 3: XIAP promotes the invasion of bladder cancer cells by inhibiting the expression of YTHDC1.
From: XIAP promotes metastasis of bladder cancer cells by ubiquitylating YTHDC1
A, D Plasmids nonsense, shYTHDC1#1, shYTHDC1#2 and shYTHDC1#3 were packed with lentivirus to infect UMUC3 (KO-XIAP) (A) and T24T (KO-XIAP) (D), and the knockdown efficiency was detected by western blotting. Transwell assay was used to detect the effects of YTHDC1 knockdown on migration and invasion ability of UMUC3 (KO-XIAP) (B) and T24T (KO-XIAP) (E). The statistical results of migration and invasion ability of YTHDC1 knockdown in UMUC3 (KO-XIAP) (C) and T24T (KO-XIAP) (F). G T24T (KO-XIAP Nonsense), T24T (KO-XIAP shYTHDC1#1) and T24T (KO-XIAP shYTHDC1#3) cells were injected into nude mice through the tail vein. The formation of lung metastases was observed 12 weeks later. H Paraffin embedding of lung tissue for HE staining analysis. I The number of lung metastases was counted.
