Fig. 4: XIAP regulates YTHDC1 ubiquitination and stability.
From: XIAP promotes metastasis of bladder cancer cells by ubiquitylating YTHDC1
UMUC3 (KO-XIAP), T24T (KO-XIAP) and control cells were pretreated with MG132 (10 μM) for 8 h (A, C), CHX (50 μg/mL) was then added at 4, 8, and 12 h and the degradation rate of YTHDC1 was detected by western blotting (B, D). E Ubiquitination assays to detect the effects of XIAP overexpression on YTHDC1 ubiquitination levels in UMUC3 cells. F Ubiquitination assays to detect the effects of XIAP knockdown on YTHDC1 ubiquitination levels in UMUC3 cells. G Ubiquitination assays to detect the effects of RING domain deletion of XIAP on YTHDC1 ubiquitination levels in UMUC3 cells. H YTHDC1 expression was detected after overexpression of XIAP-WT, 467 and 495 mutant plasmids by western blotting in UMUC3 (KO-XIAP) cells. I Detection of YTHDC1 degradation rate in HA-XIAP, HA-XIAPH467A and HA-XIAPF495A recovered UMUC3 (KO-XIAP) cells.
