Fig. 6: XIAP promotes bladder cancer invasion via the YTHDC1/MMP-2 pathway.
From: XIAP promotes metastasis of bladder cancer cells by ubiquitylating YTHDC1
A, B Western blot was used to detect the protein expression levels of MMP-2 in UMUC3/T24T (KO-XIAP shYTHDC1) cells. C MMP-2 knockdown efficiency were detected in UMUC3 (KO-XIAP shYTHDC1#3) by western blotting. D Transwell assay was used to detect the effects of MMP-2 knockdown on migration and invasion ability of UMUC3 (KO-XIAP shYTHDC1#3). E The statistical results of migration and invasion ability of MMP-2 knockdown in UMUC3 (KO-XIAP shYTHDC1#3) cells. F, G The mRNA levels of MMP-2 were analyzed in UMUC3 (KO-XIAP) (F) and T24T (KO-XIAP) (G) cells with or without YTHDC1 knockdown. H MMP-2 promoter and TK plasmids were co-transfected into UMUC3 (KO-XIAP) cells with or without YTHDC1 knockdown and luciferase activity was measured. I MMP-2 mRNA stability assay, in UMUC3 (KO-XIAP) cells with or without YTHDC1 knockdown. J MeRIP-qPCR analysis of m6A-modified MMP-2 mRNA. K RIP assay detected YTHDC1 binding MMP-2 mRNA.
