Fig. 1: Downregulation of TRMT10A in glioma cells promotes VM formation. | Cell Death & Disease

Fig. 1: Downregulation of TRMT10A in glioma cells promotes VM formation.

From: TRMT10A regulates tRNA-ArgCCT m1G9 modification to generate tRNA-derived fragments influencing vasculogenic mimicry formation in glioblastoma

Fig. 1

A The mRNA expression levels of TRMT10A in WHO grade II–IV glioma samples were analyzed using the CGGA database. Values are presented as mean ± SD, **P < 0.01 compared to WHO grade II. B Representative images of TRMT10A immunohistochemical stains in peritumoral non-glioma tissue, low-grade glioma (LGG) and high-grade glioma (HGG). Values are presented as mean ± SD (n = 5), ***P < 0.001 compared to non-neoplastic brain tissue. Scale bar = 100 μm. C The effect of TRMT10A expression levels on the survival of GBM patients was analyzed using the CGGA database. The expressions of TRMT10A in human astrocyte cell line SVG p12, and glioma cell lines U-87 MG, U-251 MG, and T98G were detected by RT-qPCR (D) and Western blot (E), respectively. Values are presented as mean ± SD (n = 3), **P < 0.01, ***P < 0.001 compared to the SVG p12 cell group. The TRMT10A knockdown levels in U-251 MG and T98G glioma cells were verified by RT-qPCR (F) and western blot (G), respectively, following the transfection of sh-TRMT10A vectors. After 48 h and 72 h of culture with stable TRMT10A knockdown U-251 MG and T98G cells, H cell proliferation was assessed using the CCK-8 assay (n = 5). Values are presented as mean ± SD, **P < 0.01 compared to the sh-NC group of 48 h; ##P < 0.01 compared to the sh-NC group of 72 h; &&P < 0.01 compared to the sh-TRMT10A group of 48 h. I Cell migration and invasion abilities were evaluated using the Transwell assay (n = 3). J The tube formation ability of cells was assessed using the tube formation assay (n = 3). Values are presented as mean ± SD, **P < 0.01 compared to the sh-NC group. Scale bar = 50 μm. K Subcellular localization of TRMT10A in U-251 MG and T98G cells was observed using laser confocal microscopy. Scale bar = 20 μm. The expressions of TRMT10A in U-251 MG and T98G cells after transfection with TRMT10A overexpression vectors were verified by RT-qPCR (L) and western blot (M), respectively. Values are presented as mean ± SD (n = 3), **P < 0.01 compared to the OE-NC group. After 48 h and 72 h of culture with stable TRMT10A overexpression in U-251 MG and T98G cells, N cell proliferation was assessed using the CCK-8 assay (n = 5). Values are presented as mean ± SD, **P < 0.01 compared to the sh-NC group of 48 h; ##P < 0.01 compared to the sh-NC group of 72 h; &P < 0.05, &&P < 0.01 compared to the sh-TRMT10A group of 48 h. O Cell migration and invasion abilities were evaluated using the Transwell assay (n = 3). Scale bar = 50 μm. P The tube formation ability of cells was assessed using the tube formation assay (n = 3). Values are presented as mean ± SD, **P < 0.01 compared to the OE-NC group. Scale bar = 50 μm.

Back to article page