Fig. 3: TRMT10A affects VM formation by regulating the expression of tRF-22. | Cell Death & Disease

Fig. 3: TRMT10A affects VM formation by regulating the expression of tRF-22.

From: TRMT10A regulates tRNA-ArgCCT m1G9 modification to generate tRNA-derived fragments influencing vasculogenic mimicry formation in glioblastoma

Fig. 3

A The expressions of tRF-22 in SVG p12, U-87 MG, U-251 MG, and T98G cells were detected by RT-qPCR. Values are presented as mean ± SD (n = 3), **P < 0.01, ***P < 0.001 compared to the SVG p12 cell group. B Subcellular localization of tRF-22 in U-251 MG and T98G cells was observed using laser confocal microscopy. Scale bar = 20 μm. C After RNA nuclear-cytoplasmic separation, the expressions of tRF-22 in the nucleus and cytoplasm of U-251 MG and T98G cells was detected by RT-qPCR. GAPDH and U6 were used as cytoplasmic and nuclear RNA markers, respectively. D After 48 h of transfection with tRF-22 mimic and inhibitor in U-251 MG and T98G cells, cell proliferation was assessed using the CCK-8 assay (n = 5). E Cell migration and invasion abilities were evaluated using the Transwell assay (n = 3). Scale bar = 50 μm. F Tube formation ability of cells was assessed using the tube formation assay (n = 3). Values are presented as mean ± SD, **P < 0.01 compared to the inhibitor NC group, ##P < 0.01 compared to the mimic NC group. Scale bar = 50 μm. G After 48 h of transfection with tRF-22 inhibitor in TRMT10A stable knockdown U-251 MG and T98G cells, cell proliferation was assessed using the CCK-8 assay (n = 5). H Cell migration and invasion abilities were evaluated using the Transwell assay (n = 3). I Tube formation ability of cells was assessed using the tube formation assay (n = 3). Values are presented as mean ± SD, **P < 0.01 compared to the sh-NC group; ##P < 0.01 compared to the sh-TRMT10A + inhibitor NC group. Scale bar = 50 μm.

Back to article page