Fig. 5: tRF-22 regulates VM formation in GBM by negatively regulating the expression of MXD1. | Cell Death & Disease

Fig. 5: tRF-22 regulates VM formation in GBM by negatively regulating the expression of MXD1.

From: TRMT10A regulates tRNA-ArgCCT m1G9 modification to generate tRNA-derived fragments influencing vasculogenic mimicry formation in glioblastoma

Fig. 5

Changes in MXD1 expression were verified by RT-qPCR (A) and western blot (B), respectively, after silencing or overexpressing MXD1 in U-251 MG and T98G cells. Values are presented as mean ± SD (n = 3), **P < 0.01, ***P < 0.001  compared to the NC group. C Cell proliferation was assessed using the CCK-8 assay after 48 h of culture (n = 5). D Cell migration and invasion abilities were evaluated using the Transwell assay after 48 h of culture (n = 3). E Tube formation ability of cells was assessed using the tube formation assay (n = 3). Values are presented as mean ± SD, **P < 0.01 compared to the sh-NC group; ##P < 0.01 compared to the OE-NC group. Scale bar = 50 μm. F After 48 h of transfection with tRF-22 inhibitor in MXD1 stable knockdown U-251 MG and T98G cells, cell proliferation was assessed using the CCK-8 assay (n = 5). G Cell migration and invasion abilities were evaluated using the Transwell assay (n = 3). Scale bar = 50 μm. H Tube formation ability of cells was assessed using the tube formation assay (n = 3). Values are presented as mean ± SD, **P < 0.01 compared to the inhibitor NC group; ##P < 0.01 compared to the tRF-22 inhibitor + sh-NC group. Scale bar = 50 μm.

Back to article page