Fig. 1: The protein expressions of NUB1 and PCNA in HCC tissues and HCC cells are negatively correlated. | Cell Death & Disease

Fig. 1: The protein expressions of NUB1 and PCNA in HCC tissues and HCC cells are negatively correlated.

From: NUB1 reduction promotes PCNA-mediated tumor growth by disturbing the PCNA polyubiquitination/NEDDylation in hepatocellular carcinoma cells

Fig. 1

A HCC tissues and adjacent nontumor tissue samples were subjected to the quantitative proteomic analysis (n = 3). B, C Determination(B) and quantification(C) of NUB1 and PCNA protein levels in 60 paired HCC tissues and adjacent nontumor tissues by immunoblotting. D Scatter plots showed a negative correlation between protein expression of NUB1 and PCNA in HCC tissues. E Representative IHC staining and quantitative analysis of NUB1 and PCNA protein expression in 60 pairs of HCC tissues and adjacent nontumor tissues. Scale bars = 200 µm and 50 µm. F Western blot assay detects NUB1 and PCNA protein expression in normal human liver HL7702 cells and six tested HCC cells (HCCLM3, Hep3B, HepG2, SMMC7721, Huh7, and MHCC97H). G Scatter plots showed a negative correlation between protein expression of NUB1 and PCNA in HCC cells. Data are represented as the mean ± SD. *p < 0.05; **p < 0.01; ***p < 0.001.

Back to article page