Fig. 5: Macrophage CXCR5 suppresses liver regeneration after PHx.

A Diagram of the experimental model of myeloid-specific LysM-Cre Cxcr5 knockout (Cxcr5 cKO) mice and littermate control (Cxcr5^F/F, F/F) in PHx mice model. B Liver/body weight ratios of Cxcr5 cKO and F/F mice at 36 h and 48 h after 2/3 PHx (n = 6). Statistical significance was made with the ANOVA test. C, D Representative immunofluorescence images of Ki67⁺HNF4α⁺ liver cells in the indicated mice at 36 h after 2/3 PHx and quantification of the percentage of Ki67⁺HNF4α⁺ cells (n = 6). Statistical significance was made with the Mann–Whitney U test. E Western blot analysis of PCNA and Cyclin D1 expression in Cxcr5 cKO and F/F livers after 2/3 PHx, and the band intensity was quantified by densitometry (n = 6). Statistical significance was made with the ANOVA test. F Relative levels of HGF mRNA transcripts in F/F and Cxcr5 cKO livers at 36 h after 2/3 PHx (n = 6). Statistical significance was made with the Mann–Whitney U test. G Diagram of the experimental model of recombinant CXCL13 treatment in Cxcr5 CKO mice. H Liver/body weight ratios of Cxcr5 cKO mice treated with and without recombinant CXCL13 after 2/3 PHx (n = 6). Statistical significance was made with the ANOVA test. I, J Representative immunofluorescence images of Ki67⁺HNF4α⁺ liver cells in the indicated mice at 36 h and quantification of Ki67⁺HNF4α⁺ cells (n = 6). Statistical significance was made with the ANOVA test. K Western blot analysis of PCNA and Cyclin D1 expression levels in liver tissues after 2/3 PHx, and the band intensity was quantified by densitometry (n = 6). Statistical significance was made with the ANOVA test. L Diagram of the experimental model of CTRL and Cxcr5 cOE mice at 2/3 PHx. M Liver/body weight ratios in CTRL and Cxcr5 cOE mice (n = 6). Statistical significance was made with the Student’s t test. N, O Representative immunofluorescence images of liver Ki67⁺HNF4α⁺ cells in CTRL and Cxcr5 cOE mice at 48 h and quantification of the percentage of Ki67⁺HNF4α⁺ cells (n = 6). Statistical significance was made with the Student’s t test. P Western blot analysis of PCNA and Cyclin D1 expression levels in CTRL and Cxcr5 cOE livers after 2/3 PHx, and the band intensity was quantified by densitometry (n = 6). Statistical significance was made with the Student’s t test. Q Relative levels of HGF mRNA transcripts in CTRL and Cxcr5 cOE livers after 2/3 PHx (n = 6). Statistical significance was made with the Mann–Whitney U test.