Fig. 8: Neutralization of CXCL13 promotes liver regeneration after PHx.

A Diagram of the experimental PHx model in which mice were treated with CXCL13 neutralization antibody (Anti-CXCL13) or Ig G. B Survival curves of mice treated with anti-CXCL13 or Ig G after lethal 85% PHx (Ig G, n = 10; Anti-CXCL13, n = 10). Statistical significance was made with the Log rank test. C Liver/body weight ratios of the mice treated with the anti-CXCL13 or Ig G at 36 and 48 h after 2/3 PHx (n = 6). Statistical significance was made with the ANOVA test. D Serum ALT and AST levels in mice treated with the anti-CXCL13 or Ig G at 36 h after 2/3 PHx (n = 6). Statistical significance was made with the Student’s t test. E, F Representative immunofluorescence images of Ki67⁺HNF4α⁺ liver cells in the indicated mice at 36 h after 2/3 PHx and quantification of the percentage of Ki67⁺HNF4α⁺ cells (n = 6). Statistical significance was made with the Student’s t test. G Western blot analysis of PCNA and Cyclin D1 expression levels in the liver tissues of the mice treated as indicated, and the band intensity was quantified by densitometry (n = 6). Statistical significance was made with the Student’s t test. H Relative levels of HGF mRNA transcripts in hepatic macrophages from mice treated as indicated at 36 h after 2/3 PHx (n = 6). Statistical significance was made with the Student’s t test. I The relative levels of HGF mRNA transcripts in livers from mice treated with anti-CXCL13 or Ig G at 36 h after 2/3 PHx (n = 6). Statistical significance was made with the Mann–Whitney U test. J Western blot analysis of pMET expression in liver tissues of mice treated as indicated after 2/3 PHx, and the band intensity was quantified by densitometry (n = 6). Statistical significance was made with the Student’s t test. K Working model of CXCL13 in liver regeneration.