Fig. 4: Smyd3 potentiates Shcbp1 and oncogenic Kras-MAPK pathways.
a Bar charts showing the top 20 enriched KEGG pathways from the 1162 genes identified in MTP12 by ChIP-seq analysis which were shown in Fig. 1i–k. b The rank of the top 50 common genes based on upregulated genes (1940) from MTP12 vs WTV by RNA-seq and 1162 genes with active transcription activities from MTP12 by ChIP sequence analysis. c The Shcbp1 binding patterns of mammary epithelial cells in both WTP12 and MTP12 mice with antibodies of H3K4me3 generated from Integrated Genome Browser (IGB). d The enrichment binding of the Shcbp1 promoter by Smyd3 antibody and IgG by ChIP-qPCR in Brca1-MT cells. e Shcbp1 protein levels in mammary tissues from WTP12, MTP12, tumors from WT and Brca1MKO mice (n = 3 mice/group) by Western blots. f The Shcbp1 expression in both B477 and G600 cells expressing sgSmyd3 and sgSmyd3/OE-Smyd3 as determined by qPCR. g The Protein levels of Smyd3, Shcbp1, pMek, and Kras in G600 cells expressing sgSmyd3 by Western blots. h Protein levels of Smyd3, Shcbp1, Kras, and pMek in Brca1-MT cells treated with the Smyd3 protein inhibitor BCI-121 at different concentrations, as shown by Western blots. i The protein levels of Brca1, Smyd3, Shcbp1, Kras, and pErk in G600 cells with OE-mBrca1 cDNA as shown by Western blots. The protein levels of SMYD3, SHCBP1, KRAS and pMEK in OE-SMYD3-231 (j) or OE-SMYD3-HCC1937 cells (k) with the expression of shSHCBP1 as shown by Western blots. l Summary for Brca1-Smyd3-Shcbp1 signaling regulation and oncogenic pathways.
