Fig. 2: Hypoxia induces the secretion of CSF3 from CAFs. | Cell Death & Disease

Fig. 2: Hypoxia induces the secretion of CSF3 from CAFs.

From: Cancer-associated fibroblasts secrete CSF3 to promote TNBC progression via enhancing PGM2L1-dependent glycolysis reprogramming

Fig. 2

A The bead-based multiplex immunoassay assay to test cytokines secreted by CAFs under normoxic and hypoxic conditions (n = 6). B ELISA assay to quantify CSF3 secreted from paired CAFs under normoxic and hypoxic conditions (n = 10). C The secretion level of CSF3 increases with the prolonged hypoxic exposure (n = 3). D The comparation of CSF3 level from CAFs and TNBC cells (n = 3). E The expression of CSF3 under normoxic and hypoxic conditions was assessed by qRT-PCR (n = 3). The HIF-1α silencing efficiency (F) and CSF3 expression level (G) were verified using qRT-PCR (n = 3). H ELISA assay to test CSF3 level after interfering HIF-1α (n = 3). I The luciferase activity of different targeted plasmids containing potential binding region (n = 3). J Dual-luciferase reporter assay to verify CSF3’ binding region for HIF-1α (n = 3). The data are presented as the mean ± SD. ns, no significance; *P < 0.05, **P < 0.01, ***P < 0.001.

Back to article page