Fig. 1: Pharmacological inhibition of PTPMT1 sensitizes HCC to ferroptosis. | Cell Death & Disease

Fig. 1: Pharmacological inhibition of PTPMT1 sensitizes HCC to ferroptosis.

From: Pharmacological targeting of the mitochondrial phosphatase PTPMT1 sensitizes hepatocellular carcinoma to ferroptosis

Fig. 1

Pharmacological inhibition of PTPMT1 in Hep 3B cells was achieved through treatment with alexidine dihydrochloride (AD, the chemical structure is shown in (A)), while cystine deprivation-induced ferroptosis was induced using Erastin and Sorafenib for 24 h. Subsequently, cell viability in each group was assessed via the CCK-8 assay (B, C). Additionally, cell death in various treatment groups (AD (1 μM) and Erastin (10 μM)) was confirmed using PI staining (D). Scale bar = 50 μm. To analyze the ferroptosis level, the expression of PTGS2 (E) and lipid peroxidation (F) were measured using qRT-PCR and BODIPY staining, respectively. Results are expressed as mean ± SD, and the P value less than 0.05 was considered statistically significant. *:P < 0.05 compared between two groups.

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