Fig. 2: AD-induced PTPMT1 inhibition contributes to the labile iron pool. | Cell Death & Disease

Fig. 2: AD-induced PTPMT1 inhibition contributes to the labile iron pool.

From: Pharmacological targeting of the mitochondrial phosphatase PTPMT1 sensitizes hepatocellular carcinoma to ferroptosis

Fig. 2

Hep 3B cells were exposed to AD at concentrations of 0, 0.5, 1, and 2 μM for 24 h. Then the cells were collected and subjected to immunoblotting to assess the expression of ferroptosis regulators (A, B). Concurrently, the intracellular concentration of free Fe2+ was quantified using FerroOrange staining (C). The protein abundance of ferritin light chain (FTL) was also evaluated across the treatment groups (D). Subsequently, the expression profiles of various autophagy makers were analyzed via western blotting (E, F), and the influence of AD treatment on autophagic flux was tested via live cell immunofluorescence assay (G). Finally, the transcription of FTL was evaluated in Hep 3B treated with AD at different concentrations (H). Data are presented as mean ± SD, and the P value less than 0.05 was considered statistically significant. *:P < 0.05 compared with Ctrl group.

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