Fig. 1: PRKCSH is upregulated in CRC samples and correlates with radioresistance.

A PRKCSH mRNA levels in 24 human colorectal cancer (CRC) samples and matched normal tissues were analyzed using NCBI GEO microarray data. Results are expressed as mean ± SD and analyzed using a two-tailed Student’s t-test (***P < 0.001). B PRKCSH mRNA expression in radiation-resistant (n = 6) and radiation-sensitive (n = 6) CRC tissues was quantified. Data are presented as mean ± SD, with statistical analysis performed using a two-tailed Student’s t-test (*P < 0.05). C Western blot analysis confirmed PRKCSH expression levels at different time points post-8 Gy irradiation in HCT116 cells, with gray value analysis shown below. D Western blot analysis confirmed PRKCSH expression levels at different time points post-8 Gy irradiation in RKO cells, with gray value analysis shown below. E Western blot analysis confirmed PRKCSH knockdown efficiency in HCT116 and RKO CRC cell lines. F, G Cell proliferation in various CRC groups post-8 Gy irradiation was evaluated using the CCK-8 assay. H, I The CCK-8 assay was performed to evaluate cell proliferation across different radiation doses, including NC and PRKCSH-KD groups. Cell viability was measured at 72 h post-irradiation. J, K The scratch wound healing assay was used to assess the migration ability of HCT116 cells, including NC and PRKCSH-KD groups, at 24 h post-irradiation. L, M The Transwell invasion assay was conducted to evaluate the invasive capacity of HCT116 cells, including NC and PRKCSH-KD groups, at 24 h post-irradiation. Data represent mean ± SD from three independent experiments. Error bars indicate SD. Statistical significance: *P < 0.05, **P < 0.01, ***P < 0.001.