Fig. 2: Knockdown of PRKCSH improves radiosensitivity of CRC cells.
From: PRKCSH enhances colorectal cancer radioresistance via IRE1α/XBP1s-mediated DNA repair
A–C Representative images and quantitative analysis of colony formation survival assays were performed on PRKCSH knockdown (PRKCSH-KD) and negative control (NC) cells following radiation at 0, 2, 4, and 6 Gy. D–F Apoptosis in colorectal cancer cells from different groups was assessed by flow cytometry at 24 and 48 h post-8 Gy radiation. In (E, F) apoptotic cells include both early and late apoptotic cells, as determined by Annexin V-APC positivity. G Western blot analysis detected the expression of apoptosis-related proteins in NC and PRKCSH-KD cells at various time points post-8 Gy radiation. H The relative Bcl2/Bax ratio was evaluated. Data are presented as mean ± standard deviation from three independent experiments. Error bars indicate standard deviation. Statistical significance is indicated as *P < 0.05, **P < 0.01, ***P < 0.001.
