Fig. 3: Knockdown of PRKCSH inhibits radiation-induced ER stress response.
From: PRKCSH enhances colorectal cancer radioresistance via IRE1α/XBP1s-mediated DNA repair
A Representative images of Western Blot of the protein expression in IRE1α/XBP1s pathway in HCT116 cells after 8 Gy IR at different time points. B, D Quantitative analysis of GRP78, p-IRE1α, IRE1α, and XBP1s protein levels. E Relative expression of DGAT2 mRNA in HCT116 cells at different time points post-irradiation. F Representative images of Western Blot of the protein expression in UPR pathway in NC and PRKCSH-KD cells after 8 Gy IR at different time points. G Relative OD values at 450 nm for HCT116 cells after 8 Gy irradiation, treated with NC, PRKCSH-KD, PRKCSH-KD + Vector, PRKCSH-KD + XBP1s, and the IRE1 inhibitor ATF-083010 (25 μM and 50 μM). Data are representative of three independent experiments with similar results. Error bars, SD. **P < 0.01, ***P < 0.001.
