Fig. 4: Knockdown of PRKCSH attenuates the radiation-induced DNA damage repair response.
From: PRKCSH enhances colorectal cancer radioresistance via IRE1α/XBP1s-mediated DNA repair
A Comet assay images depict the conditions of NC and KD cells 8 h after exposure to 8 Gy IR. B, C Tail moments in NC and KD cells were quantified using CASP 1.2.3b2 software. D Immunofluorescence staining was employed to detect γ-H2AX foci in HCT116 and RKO cells following 8 Gy IR. E, F The number of γ-H2AX foci per cell was quantified in HCT116 (E) and RKO (F) cells, with 30 cells per group at each time point. G Western blot images show the expression of DNA damage response and cell cycle proteins in HCT116-NC and HCT116-KD cells at various time points post-8 Gy IR. H, I The cell cycle distribution in HCT116 cells, treated with NC and PRKCSH-KD, was assessed at 0 and 24 h post-irradiation. Data are from three independent experiments and are presented with SD error bars. **P < 0.01, ***P < 0.001.
