Fig. 2: SPHK1 knockdown has a more pronounced effect than TPKI knockdown on the growth and survival fate of K562 cells. | Cell Death & Disease

Fig. 2: SPHK1 knockdown has a more pronounced effect than TPKI knockdown on the growth and survival fate of K562 cells.

From: Dysregulation of sphingolipid metabolism contributes to the pathogenesis of chronic myeloid leukemia

Fig. 2

A Diagram showing the sphingosine and thiamine metabolism pathways. B Validation of SPHK1 and TPK1 shRNA-mediated knockdown efficiencies in K562 cells using qRT-PCR (n = 3). C Validation of SPHK1 and TPK1 shRNA-mediated knockdown efficiencies in K562 cells using Western blot. D Detection of cell growth ability after SPHK1 or TPK1 knockdown (n = 3). E Flow cytometric analysis of cellular apoptosis after SPHK1 or TPK1 knockdown (n = 3). The bar graph data in Fig. 2 are presented as mean ± S.E.M.

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