Fig. 1: RIPK2 is identified as an independent risk factor for poor prognosis of CRC. | Cell Death & Disease

Fig. 1: RIPK2 is identified as an independent risk factor for poor prognosis of CRC.

From: RIPK2 promotes colorectal cancer metastasis by protecting YAP degradation from ITCH-mediated ubiquitination

Fig. 1

A–C Volcano plot of DEGs identified from GSE22598, GSE39582 and TCGA (COAD and READ) datasets. Red pots express upregulated DEGs, and blue pots express downregulated DEGs under the same thresholds. The gray dots denote genes that are not differentially expressed. |log2FC | > 1.0 and adjusted P value < 0.05 were set as the cut-off criteria. D The intersection of differentially protein kinase genes of GSE22598, GSE39582, TCGA (COAD and READ) datasets and KinBase database. E Seven differentially expressed protein kinase genes (AURKA, BUB1, CDK4, MET, NEK2, RIPK2, TRIB3) at mRNA levels were examined between tumor and paired adjacent normal tissues using the GSE41258 dataset. F Four of seven genes (AURKA, BUB1, NEK2, TRIB3) were not existed in CPTAC database (PDC000116), the other genes (CDK4, MET, RIPK2) at protein levels were assessed between tumor and paired adjacent normal tissues. Overall survival analysis of MET (G) and RIPK2 (H) in CRC cohorts, including GSE39582, GSE41258, GSE87211 and TCGA (COAD and READ), using Kaplan-Meier survival analysis. I Univariate and multivariate Cox proportional regression analyses for OS in CRC cohorts was visualized by forest plot. J RIPK2 mRNA expression levels in 40 CRC primary tumor tissues and paired normal tissues were detected using RT-qPCR. K Expression analysis grouped by tumor stage of RIPK2 in 40 CRC cases were performed using RT-qPCR. L Representative images of IHC slides showed RIPK2 protein levels in CRC tumor tissues and normal tissues. M Pie chart figure showed the distribution of cases with RIPK2 protein levels, containing negative (–), weakly positive (+), moderately positive (++), and strongly positive (+++). N RIPK2 protein expression levels in 6 CRC primary tumor tissues and paired normal tissues were detected using western blotting. Kaplan-Meier survival analysis estimated the relationship between RIPK2 protein levels and OS (O) or RFS (P) in 152 CRC cases. *<0.05, **<0.01, ***<0.001, ****<0.0001.

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