Fig. 3: ST mapping and spatial trajectory analysis of RIPK2+ tumor cell cluster. | Cell Death & Disease

Fig. 3: ST mapping and spatial trajectory analysis of RIPK2+ tumor cell cluster.

From: RIPK2 promotes colorectal cancer metastasis by protecting YAP degradation from ITCH-mediated ubiquitination

Fig. 3

The distribution map of clusters was generated using Louvain clustering and spot feature analysis methods in the primary lesion (A) and liver metastasis lesion (F). The spatial expression distribution map was generated using SPOTlight to predict the possible spatial distribution characteristics of the RIPK2+ tumor cell cluster (C) and the low-malignancy tumor epithelial cell cluster, cluster 3 (B). D Potential spatial differentiation trajectory from cluster 3 to the RIPK2+ tumor cell cluster. E Trajectory analysis of the RIPK2+ cluster over pseudotime, with different cell types distinguished by color (Left) and pseudotime indicated by color intensity (Right). The spatial distribution characteristics of RIPK2+ tumor cell cluster (G) and RIPK2 expression (H) were predicted using SPOTlight in the liver metastasis lesion. I Representative IHC images of RIPK2 in liver metastasis samples.

Back to article page