Fig. 7: GSK583 inhibits CRC metastasis and promotes YAP degradation by K48-linked ubiquitination. | Cell Death & Disease

Fig. 7: GSK583 inhibits CRC metastasis and promotes YAP degradation by K48-linked ubiquitination.

From: RIPK2 promotes colorectal cancer metastasis by protecting YAP degradation from ITCH-mediated ubiquitination

Fig. 7

A Transwell assay of CRC cells treated with DMSO or GSK583. B Luminescence images (Right) and bar plot (Left) of mice treated with GSK583, compared with control treatment. C H&E staining (Left) and Bar plot (Right) of measuring effect of GSK583 treatment on the number of pulmonary metastases in mice. D HCT116 cells were treated with MDP for 2 h in the presence or absence of GSK583 (added 2 h prior). Their lysates were immunoprecipitated with indicated RIPK2 antibody, and precipitates and lysates were examined by western blotting using indicated antibodies. E Representative immunoblots of the indicated proteins of total lysates from HCT116 (left) and SW480 (right) cells treated with 10 μM GSK583 at 1 h, 2 h, and 4 h, compared with control cells treated with DMSO. F Ubiquitin (linkage-specific K48) levels in total lysates of CRC cells transfected with HA-K48 plasmids and treated with GSK583 and MG132, compared with control cells treated with DMSO and MG132. G YAP protein was immunoprecipitated from CRC cells transiently transfected with HA-K48 plasmids and treated with GSK583 and MG132. Immunoprecipitation product were analyzed by western blotting.

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