Fig. 1: NDP52^GE binds LC3C more efficiently than NDP52^WT in a human neuroblastoma cell line.

A Lysates of SH-SY5Y cells expressing the indicated FLAG- and HA-tagged proteins was immunoprecipitated with anti-HA beads. Samples were analyzed by Western blot using the indicated antibodies. The graph reports the amount of the indicated FLAG-NDP52 protein coprecipitated by the corresponding HA-LC3C protein. Data were expressed as percentage variation over FLAG-NDP52^WT. Images and data are representative of seven independent experiments. B Lysates of SH-SY5Y cells expressing the indicated GFP- and HA-tagged proteins were immunoprecipitated with anti-GFP beads. Samples were analyzed by Western blot using the indicated antibodies. The graph reports the amount of HA-LC3C coprecipitated by the corresponding GFP-NDP52 protein. Data were expressed as percentage variation over GFP-NDP52^WT. Images and data are representative of four independent experiments. C Representative confocal images of SH-SY5Y cells expressing the indicated GFP- and HA-tagged proteins, fixed and stained with anti-HA (magenta staining) antibodies and with DAPI (blue staining in the merge panels) to detect nuclei. Colocalization of GFP-NDP52 and HA-LC3C was quantified by measuring the Pearson’s Correlation Coefficient. The results are reported in the graph. Images are representative of three independent experiments, and at least 30 cells for each condition were analyzed. Scale bar: 10 μm. In (B, C) data are presented as means ± SEM. *p < 0,05; ***p = <0.001 (two-tailed unpaired t-test).