Fig. 5: hNDP52^GE improves hTAU phenotypes in Drosophila melanogaster better than hNDP52^WT.

A Lysates from heads of flies expressing the indicated transgenes in the eye, at 29 °C, under control of GMR-GAL4 driver were analyzed by WB using the indicated antibodies. + refers to non-transgenic flies used as control. Images are representative of four independent experiments. The ratio between dAtg8II/dAtg8I was calculated and reported in the graph as means ± SEM. * indicates comparison of dAtg8II/dAtg8I between flies expressing hNDP52^GE and control; § indicates comparison of dAtg8II/dAtg8I between flies expressing hNDP52^GE and hNDP52^WT (Ordinary One-way ANOVA, Turkey’s multiple comparisons test). B Representative images of eye from flies expressing the indicated human (h) transgenes at 25 °C under control of eyeless-GAL4 driver. Violin plot below reports eye areas. Median and quartiles are shown as dotted lines. More than 150 eyes from both males and females flies were measured for each condition. * indicates comparison to control (+); # indicates comparison to hTAU; § indicates comparison to hTAU;hNDP52^WT (Ordinary One-way ANOVA, Turkey’s multiple comparisons test). C Box and whiskers (min to max) plot showing the percentage of flies reaching the threshold. Female flies expressed panneuronally (elav-GAL4) the indicated human transgenes at 29 °C. More than 40 flies per genotype were analyzed (Control n = 54 flies; hTAU n = 44; hTAU, NDP52^WT n = 53; hTAU, NDP52^GE n = 48 flies). * indicates comparison to control (+); # indicates comparison to hTAU (Ordinary One-way ANOVA, Turkey’s multiple comparisons test). D Survival curve showing lifespan of female flies expressing panneuronally (elav-GAL4) the indicated transgenes at 29 °C. * indicates comparison to control (+); # indicates comparison to hTAU (Log-rank (Mantel-Cox) test). E Lysates from heads of flies expressing panneuronally (elav-GAL4) the indicated transgenes at 29 °C were analyzed by WB using the indicated antibodies. Images are representative of four independent experiments. Signals from the indicated TAU antibodies were measured and normalized to the corresponding signal of GIOTTO. Results are expressed as means ± SEM. # indicates comparison to hTAU; § indicates comparison to hTAU;hNDP52^WT (Ordinary One-way ANOVA, Turkey’s multiple comparisons test).