Fig. 1: Integrative analysis of stemness and immune characteristics in the transcriptome of gastric cancer patients.

A The integrated heatmap illustrates the stemness pathway scores for each patient across the two stemness phenotypes. B Student’s t test was used to evaluate the differences in stemness pathway scores between the two stemness subgroups. The upper and lower edges of the box represent the interquartile range, the line within the box denotes the median, and the dots indicate outliers. C GO enrichment analysis illustrated the pathway enrichment profiles within the two stemness subgroups. D The integrated heatmap displays the immune cell infiltration profiles for each patient across the two immune phenotypes. E Student’s t test was used to assess differences in immune cell infiltration between the two immune subgroups. The upper and lower edges of the box represent the interquartile range, the line within the box indicates the median, and the dots denote outliers. F GO enrichment analysis revealed pathway enrichment profiles across the two immune subgroups. G Venn diagram illustrates the overlap between differentially expressed genes associated with the stemness subgroups and those associated with the immune subgroups. The intersecting differentially expressed genes were further analyzed using Weighted Gene Co-Expression Network Analysis (WGCNA). H The Module–Trait Relationships diagram illustrates the correlations between different modules and the clinical information of neoadjuvant-treated patients. *p < 0.05; **p < 0.01; ***p < 0.001.