Fig. 1: MTCH2 is highly expressed in melanoma and is related to the degree of malignancy of melanoma.

A The expression of MTCH2 in normal tissue and melanoma tissue based on GEPIA database. B The relationship of MTCH2 with survival rate in melanoma based on TCGA data. C, D The mRNA and protein expression of MTCH2 in HEM cell and melanoma cells were analyzed by qPCR (C) and western blotting analysis (D). E–H Representative images of MTCH2 expression in the melanoma and the normal tissue microarray are shown. Scale bars = 50 μm (E). Immunohistochemical results of MTCH2 expression in melanoma tissue microarray (F). The percentage of high and low expression of MTCH2 in normal tissues, primary, and metastatic melanoma (G). MTCH2 score (H). I–L The stable MTCH2 knockdown and overexpression in A375 and SK-MEL-21 cells were established. The expression of MTCH2 were measured by western blotting (I, J) and qPCR (K, L) analysis. The data represent three independent experiments. The data as indicated the mean ± SD and was analyzed by Student’s t test. (*p < 0.05; **p < 0.01; ***p < 0.001).