Fig. 2: MTCH2 promotes melanoma cells proliferation in vitro and vivo. | Cell Death & Disease

Fig. 2: MTCH2 promotes melanoma cells proliferation in vitro and vivo.

From: MTCH2 regulates NRF2-mediated RRM1 expression to promote melanoma proliferation and dacarbazine insensitivity

Fig. 2

A, B The proliferation rate of MTCH2 knockdown or MTCH2 overexpressing A375, SK-MEL-21 and the corresponding control cells were detected by MTS assay. C EdU was used to evaluate the proliferation of MTCH2 knockdown or MTCH2 overexpressing A375 and SK-MEL-21 cells. Scale bars = 200 μm. D The phase distribution of MTCH2 knockdown or MTCH2 overexpressing A375 and the corresponding control cells cell cycle were detected by flow cytometry. E Western blot was used to detect the protein expression of Cyclin E1 and CDK2 in MTCH2 knockdown or MTCH2 overexpressing A375 and SK-MEL-21 cells. F BALB/c nude mice were injected with MTCH2 knockdown or MTCH2 overexpressing A375 cells and the control cells. Measure tumor volume. (n = 4). G Morphological feature of each xenograft tumor group by HE staining and IHC. Scale bars = 50 μm. The data represent three independent experiments. Each bar represents mean ± SD. p values were calculated using a Student’s t test (*p < 0.05, **p < 0.01, ***p < 0.001 vs. each control).

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