Fig. 7: Targeting RRM1 promoted the antitumor activity of dacarbazine in vitro and in vivo. | Cell Death & Disease

Fig. 7: Targeting RRM1 promoted the antitumor activity of dacarbazine in vitro and in vivo.

From: MTCH2 regulates NRF2-mediated RRM1 expression to promote melanoma proliferation and dacarbazine insensitivity

Fig. 7

A–F Relative cell viability of A375 and SK-MEL-21 cells with MTCH2 knockdown (A, B), MTCH2 overexpressing (C, D) and RRM1 knockdown (E, F) treated with different concentrations of dacarbazine for 24 h. G BALB/c nude mice were injected with A375 cell that were stably transfected with RRM1 knockdown and the control. After one week of tumor injection, start treatment with dacarbazine. Representative images of mice with RRM1 control and knockdown xenograft tumors. The tumor volume (H) and the tumor weight (I) were measured. (n = 5/group). J Morphological feature of each xenograft tumor group by HE staining and IHC. The data represent three independent experiments. Scale bars = 50 μm. The data represent three independent experiments. The data related to tumor volume and weight, IHC were statistically analyzed by two-way ANOVA (*p < 0.05, **p < 0.01, ***p < 0.001).

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