Fig. 2: PGC1α- non-cell autonomous anti-proliferative effect is dependent on ERRα and restricted to the soluble fraction of the conditioned media. | Cell Death & Disease

Fig. 2: PGC1α- non-cell autonomous anti-proliferative effect is dependent on ERRα and restricted to the soluble fraction of the conditioned media.

From: Secreted spermidine synthase reveals a paracrine role for PGC1α-induced growth suppression in prostate cancer

Fig. 2

A Quantification of 2D-cell proliferation (crystal violet) of PC3 (n = 3) grown with differential conditioned media produced by PGC1α non-expressing and expressing PC3 cells with or without deletion of ERRα. A representative image of the crystal violet staining is included the quantifications. B Quantification of 2D-cell proliferation (crystal violet) of PC3 (n = 3) grown with the heavy fraction of conditioned media (>10 kDa) produced by PGC1α non-expressing and expressing PC3 cells with or without deletion of ERRα. C Effect of EVs produced by PGC1α non-expressing and expressing PC3 cells on the 2D-cell proliferation (crystal violet) of PC3 cells during 5 (left panel, n = 4) and 7 days (right panel, n = 5). D Effect of EVs-depleted fraction produced by PGC1α non-expressing and expressing PC3 cells on the 2D-cell proliferation (crystal violet) of PC3 cells during 5 (left panel, n = 4) and 7 days (right panel, n = 4). All data are normalized to the CM - Dox (non-PGC1α expressing) condition, depicted by a black dotted line. R: recipient cells. CM conditioned media. SFs soluble factors, EVs extracellular vesicles. D or Dox: doxycyline. Statistics: one sample t-test with reference value 1 (A–D); paired-t-test (A, B). */$ p.value 0.05; **/$$ p.value < 0.01; ***/$$$ p.value 0.001. Asterisks indicate statistical difference between No Dox and Dox conditions and dollar symbols indicate statistical difference between Control Dox and sgERRα#1/sgERRα#2 Dox. Error bars indicate s.e.m.

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