Fig. 2: VRK1 promotes the proliferation and migration of liver cancer cells.

A The expression of VRK1 in normal liver cells and liver cancer cells. B Diagram of VRK1 shRNA targeting region in VRK1 coding sequence. Immunoblots analysis of VRK1 protein level (C) and qRT-PCR analysis of VRK1 mRNA level (D) in Huh7 cells infected with VRK1 shRNA. CCK8 (E), colony formation (F, G) and transwell assay (H, I) were determined in Huh7 cells. Data are represented as means ± SD relative to the control group (n = 3). *p < 0.05, **p < 0.01.