Fig. 4: SLC25A42 inhibits ferroptosis in GC cells. | Cell Death & Disease

Fig. 4: SLC25A42 inhibits ferroptosis in GC cells.

From: SLC25A42 promotes gastric cancer growth by conferring ferroptosis resistance through enhancing CPT2-mediated fatty acid oxidation

Fig. 4

A Flow cytometry was utilized to investigate the rates of cell death of GC cells treated with specific inhibitors targeting different types of programmed cell death. 3-Methyladenine (3-MA) targeting autophagy, Z-VAD-FMK (ZVF) targeting apoptosis, Necrostatin-1 (NEC-1) targeting necroptosis. The levels of lipid peroxidation (B) and intracellular Fe2+ (C) were assessed in GC cells exhibiting either SLC25A42 knockdown or overexpression. Scale bar, 10 μm. D IHC staining of 4-hydroxy-2-noneal (4-HNE) in xenograft tumor tissues developed from SLC25A42 knockdown or control MKN-1 cells. F Flow cytometry was utilized to investigate the rates of apoptosis of GC cells. The levels of lipid peroxidation (E) and intracellular Fe2+ (F) were assessed in GC cells treated with the ferroptosis inducer RSL3. Scale bar, 10 μm. *P < 0.05.

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