Fig. 1: YTHDF1 is highly expressed in prostate cancer.

A The expression levels of YTHDF1 across various tumor types were analyzed utilizing data from the TCGA database. B Analysis from the TNMplot website indicated that YTHDF1 expression in prostate cancer tissues was elevated. C. A matched expression analysis of prostate cancer patients using the TCGA database revealed that YTHDF1 was significantly overexpressed in prostate cancer. D Prostate cancer patients exhibiting high YTHDF1 expression were associated with a poorer prognosis. E The Receiver Operating Characteristic (ROC) curve analysis of YTHDF1 revealed an Area Under the Curve (AUC) value of 0.749. F, G YTHDF1 expression positively correlates with elevated tumor stages. H, I Tissue microarray staining demonstrated a markedly high expression of YTHDF1 in cancerous tissues. J, K YTHDF1 exhibited high expression in prostate cancer cell lines at both the mRNA (J) and protein (K) levels. L, M Assessment of YTHDF1 knockdown efficiency in DU145 and 22RV-1 cell lines was conducted, with (L) representing mRNA levels and (M) representing protein levels. N The knockdown of YTHDF1 resulted in the inhibition of proliferation in prostate cancer cell lines. O-P The knockdown of YTHDF1 led to a reduction in colony formation efficiency, with (P) providing the statistical analysis of the results observed in (O). Means ± SEM, *P < 0.05; **P < 0.01; ***P < 0.001; t-test. Ctr =Control shRNA or scrambled shRNA; YTH1 = YTHDF1; sh=shRNA; sh#1=shRNA#1; sh#2=shRNA#2.