Fig. 6: Neddylation inhibitor MLN4924 repressed tumor growth.

A The IC₅₀ of indicated cell lines treated with different concentrations of MLN4924. (MLN = MLN4924). B Representative images for the colony assay in DU145 and 22RV-1 cells treated with indicated dosages of MLN. C The growth curve of DU145 and 22RV-1 cells treated with indicated dosages of MLN. The proteins (D) and mRNAs (E) of p27 and RNF7 in DU145 cells treated with indicated dosages of MLN were examined by immunoblot and qRT-PCR, respectively. F The ubiquitination of p27 after being treated with MLN (0.2 µM) was analyzed in the specified cell lines using immunoblot with indicated antibodies. G The cell viability assay for indicated cells treated with vehicle or MLN (0.2 μM) in combination with indicated dosages of DDP. H–J DU145 cells treated with or without MLN (0.2 µM) in combination with or without DDP (30 µM) were examined by flow cytometry assay (H, I) and immunoblot (J). I is the statistical result for (H). K The schematic picture of the xenograft mouse model treated with vehicle, MLN (60 mg/kg), DDP (7 mg/kg), or MLN and DDP. L–N Representative xenograft tumor mass images (L), tumor volume (M), and tumor masses (N). Means ± SEM, *P < 0.05; **P < 0.01; ***P < 0.001; t-test.