Fig. 1: TRPM7 kinase domain deletion characterization in PDAC cell lines. | Cell Death & Disease

Fig. 1: TRPM7 kinase domain deletion characterization in PDAC cell lines.

From: The kinase domain of TRPM7 interacts with PAK1 and regulates pancreatic cancer cell epithelial-to-mesenchymal transition

Fig. 1

A Schematic representation of CRISPR-Cas9 model deletion of TRPM7 kinase domain in PANC-1 and MIA PaCa-2 cell lines. B mRNA levels of TRPM7 mRNA using primers targeting the kinase domain and the channel in PANC-1 (n = 8, p < 0.05) and MIA PaCa-2 (n = 12, p < 0.05) cells (Two-way ANOVA followed by Šidák’s post-hoc test). C Protein levels of full-length TRPM7 showing a decrease of ~55% in PANC-1 (n = 7, p < 0.001), and a decrease of ~70% in MIA PaCa-2 (n = 7, p < 0.001) using a TRPM7 antibody raised against the kinase domain (Mann–Whitney test). D Electrophysiological recordings of TRPM7 currents in PANC-1 cells (Control, n = 8; ΔK, n = 12) and MIA PaCa-2 cells (Control, n = 5; ΔK, n = 12) and quantification of MIC currents at +100 mv (Mann–Whitney test). E Constitutive entry of divalent cations estimated by manganese quench in PANC-1 (Control, n = 107; ΔK, n = 117) and MIA PaCa-2 (Control, n = 176; ΔK, n = 185) cells (Mann–Whitney test). Results are shown as mean ± SD. *p < 0.05; ***p < 0.001.

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