Fig. 2: TRPM7 kinase domain deletion modifies MgATP sensitivity of MIC currents without effect on total current densities. | Cell Death & Disease

Fig. 2: TRPM7 kinase domain deletion modifies MgATP sensitivity of MIC currents without effect on total current densities.

From: The kinase domain of TRPM7 interacts with PAK1 and regulates pancreatic cancer cell epithelial-to-mesenchymal transition

Fig. 2

A Electrophysiological recordings of TRPM7 currents in PANC-1 Control and ΔK cells with 0 µM (Control, n = 8; ΔK, n = 12), 210 µM (Control, n = 7; ΔK, n = 6), 850 µM (Control, n = 5; ΔK, n = 5) and 1200 µM (Control, n = 5; ΔK, n = 4) of Mg2+ in intrapipette solution and quantification of MIC currents at +100 mV (*p < 0.05; **p < 0.01, Two-way ANOVA followed by Šidák’s post-hoc test). B Electrophysiological recordings of TRPM7 currents in MIA PaCa-2 Control and ΔK cells with 0 µM (Control, n = 15; ΔK, n = 12), 210 µM (Control, n = 5; ΔK, n = 5) and 850 µM (Control, n = 3; ΔK, n = 3) of Mg2+ in intrapipette solution and quantification of MIC currents at +100 mV (*p < 0.05, Two-way ANOVA followed by Šidák’s post-hoc test). C Electrophysiological recordings of TRPM7 currents in PANC-1 control cells containing 210 µM free Mg2+ (n = 7) or containing 210 µM free Mg2+ and MgATP (n = 5; *p < 0.05) and in ΔK cells containing 210 µM free Mg2+ (n = 6) or containing 210 µM free Mg2+ and MgATP (n = 5). Below: quantification of MIC currents at +100 mV (Two-way ANOVA followed by Šidák’s post-hoc test). D Same experiments realized in MIA PaCa-2 control cells containing 210 µM free Mg2+ (n = 5) or containing 210 µM free Mg2+ and MgATP (n = 5; p < 0.05), and in ΔK cells containing 210 µM free Mg2+ (n = 5) or containing 210 µM free Mg2+ and MgATP (n = 6). Below: quantification of MIC currents at +100 mV (Two-way ANOVA followed by Šidák’s post-hoc test). Results are shown as mean ± SD. *p < 0.05; **p < 0.01.

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